Volume 9, number 2

Enzymatic Synthesis of Ampicillin by Immobilized Penicillin G acylase of E.coli

S.M. Atyabi1, S. Hatami Gigloo1, M. Salimi2, S.B. Momen1, A. Akbarzadeh1 and D. Nrouzian1*

1Department of Pilot Biotechnology, Pasteur Institute of Iran, Tehran 13164, Iran.

2Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran13164, Iran.

Corresponding Author E-mail: dnsa@pasteur.ac.ir

DOI : http://dx.doi.org/http://dx.doi.org/10.13005/bbra/1050

ABSTRACT: Penicillin G acylase immobilized on to iron oxide nano particle coated with polyethyleneimine was used to synthesize b-lactam antibiotic namely ampicillin through kinetically controlled process. 6-Aminopenicillanic acid (6-APA) and R- phenylglycine methyl ester (PGME) were employed at different molar ratios to synthesis ampicillin batchwise. Lower molar ratio of b-lactam nucleus to higher molar ratio of acyl donor gave highest selectivity either in absence or presence of methanol at 35°C rather than 4°C. Both the selectivity and yields were time dependent. The immobilized enzyme was operationally stable for 6 continuous cycles.

KEYWORDS: Immobilized penicillin G acylase; Selectivity; Yield; Ampicillin.

Copy the following to cite this article:

Atyabi S. M, Gigloo S. H, Salimi M, Momen S. B, Akbarzadeh A, Nrouzian D. Enzymatic Synthesis of Ampicillin by Immobilized Penicillin G acylase of E.coli. Biosci Biotech Res Asia 2012;9(2)
Copy the following to cite this URL:

Atyabi S. M, Gigloo S. H, Salimi M, Momen S. B, Akbarzadeh A, Nrouzian D. Enzymatic Synthesis of Ampicillin by Immobilized Penicillin G acylase of E.coli. Biosci Biotech Res Asia 2012;9(2). Available from: https://www.biotech-asia.org/?p=10029

[ HTML Full Text]

Back to TOC