Effectiveness of ISSR Technique in Revealing Genetic Diversity of Phaseolus vulgaris L. Representing Various Parts of the World

Mohamed S. El-Shikh^1,3, Abbas A. El-Ghamery¹ and Abd El-Zaher M. A. Mustafa^2,3

1Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Egypt. 2Botany and Microbiology Department, Faculty of Science, Tanta University, Egypt. 3Botany and Microbiology Department, Faculty of Science, King Saud University, Riyadh, Saudi Arabia.

DOI : http://dx.doi.org/10.13005/bbra/1107

ABSTRACT: Phaseolus vulgaris L. is the world’s second most important bean after soybeans; used for human food and animal feed. It has generally been linked to reduced risk of cardiovascular disease, diabetes mellitus, obesity, cancer and diseases of digestive tract. The effectiveness of ISSR in achievement of the genetic diversity among 60 common bean accessions; represent various germplasms around the world was investigated. In general, the studied Phaseolus vulgaris accessions were divided into 2 major groups. All of the South-American accessions were separated into the second major group. These accessions may have different genetic features that are distinct from the rest of the accessions clustered in the major group. Asia and Europe accessions (1-20) seem to be more genetically similar (99%) to each other as they clustered in the same sub-group. The American and African varieties showed similarities as well and clustered in the same sub-tree group. In contrast, Asian and American accessions No. 22 and 23 showed high level of genetic similarities, although these were isolated from different regions. The phylogenetic tree showed that all the Asian accessions (along with Australian No. 59 and 60) were similar except Indian and Yemen accessions No. 9 and 20. Only Netherlands accession No. 3 was different from the rest of European accessions. Morocco accession No. 52 was genetically different from the rest of the African accessions. Canadian accession No. 44 seems to be different from the other North American accessions including Guatemala, Mexico and USA.

KEYWORDS: Phaseolus vulgaris L; ISSR technique; Genetic diversity

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