Volume 13, number 4
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Electrophoretic Analysis of Seed Proteins of Rice Varieties

Innabat A. Sartbayeva1,2, Bakdaulet N. Usenbekov1, Aiman B. Rysbekova1, Kulpash M. Bulatova3, Dauren T. Kazkeyev1, Eldos A. Zhanbyrbaev1, Horlan A. Berkimbay1 and Kabyl Zh. Zhambakin1

1Institute of Plant Biology and Biotechnology (Almaty 050040, Kazakhstan).

2Al-Farabi Kazakh National University (Almaty 050040, Kazakhstan).

3LLP Kazakh Scientific and research Institute of Land Farming and Plant Growing, Almalybak.

Corresponding Author E-mail: bakdaulet7@yandex.ru

DOI : http://dx.doi.org/10.13005/bbra/2349

ABSTRACT: Composition of seed storage proteins were evaluated in rice collection materials using electrophoresis in alkaline medium at presence of SDS. Optimal conditions for protein extraction and fractionation were determined, the classification of protein components spectrum and methods for composition protein formulation was developed. It was conducted the registration and clustering of 62 rice samples, of which 5 were heterogeneous in gel electrophoresis of electropherograms.

KEYWORDS: rice; SDS-PAGE; storage protein; relative electrophoretic mobility

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Sartbayeva I. A, Usenbekov B. N, Rysbekova A. B, Bulatova K. M, Kazkeyev D. T, Zhanbyrbaev E. A, Berkimbay H. A, Zh. Zhambakin K. Electrophoretic Analysis of Seed Proteins of Rice Varieties. Biosci Biotech Res Asia 2016;13(4).
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Sartbayeva I. A, Usenbekov B. N, Rysbekova A. B, Bulatova K. M, Kazkeyev D. T, Zhanbyrbaev E. A, Berkimbay H. A, Zh. Zhambakin K. Electrophoretic Analysis of Seed Proteins of Rice Varieties. Biosci Biotech Res Asia 2016;13(4). Available from: https://www.biotech-asia.org/?p=16584

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