Volume 13, number 4
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Additional Property of Xenorhabdus Stockiae for Inhibiting Cow Mastitis-Causing Bacteria

Prapassorn Bussaman1 and Paweena Rattanasena2*

1Biocontrol Research Unit, Department of Biotechnology, Faculty of Technology, Mahasarakham University, Maha Sarakham 44150, Thailand.

2Community Public Health Sub-Department, Department of Applied Sciences, Faculty of Science and Technology, Rajabhat Phranakhon Si Ayutthaya University, Ayutthaya 13000, Thailand.

Corresponding Author E-mail: paweenajay@hotmail.com

DOI : http://dx.doi.org/10.13005/bbra/2342

ABSTRACT: Xenorhabdus bacteria isolated from entomopathogenic nematodes have been found to produce several antimicrobial agents for inhibiting pathogenic bacteria. In this study, X. stockiae strains PB09, SS7 and UV58 (UV-mutant of PB09) were evaluated for antibacterial activities against cow mastitis-causing isolates of Bacillus subtilis, Staphylococcus aureus, Staphylococcus intermedius, Streptococcus agalactiae, Enterococcus faecalis, Klebsiella pneumoniae, and Escherichia coli. The paper disc diffusion showed that cell-free supernatants of all X. stockiae strains cultivated for 48, 72 and 96 h had anti-mastitis activities, but 72- and 96-h supernatants were more effective and tended to inhibit gram-positive bacteria. The maximal activities were found when X. stockiae PB09 were evaluated against S. agalactiae (12.00±2.16 mm), X. stockiae SS7 and X. stockiae UV58 against S. aureus (11.75±1.71 mm and 12.50±0.58 mm, respectively). The overlay assay also showed that X. stockiae colonies were more likely to inhibit gram-positive bacteria. Also, live X. stockiae UV58 colonies were more effective than X. stockiae PB09 and X. stockiae SS7 colonies for suppressing S. intermedius, S. agalactiae and E. coli (31.33±1.53, 22.67±1.53, 18.33±0.58 mm, respectively). This study may suggest that X. stockiae can be used for future effective suppression of mastitis infection in dairy cows.

KEYWORDS: Xenorhabdus stockiae; cow mastitis bacteria; paper disc diffusion assay; overlay assay

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Bussaman P, Rattanasena P. Additional Property of Xenorhabdus Stockiae for Inhibiting Cow Mastitis-Causing Bacteria. Biosci Biotech Res Asia 2016;13(4).
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Bussaman P, Rattanasena P. Additional Property of Xenorhabdus Stockiae for Inhibiting Cow Mastitis-Causing Bacteria. Biosci Biotech Res Asia 2016;13(4). Available from: https://www.biotech-asia.org/?p=16723

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