Antibacterial Effect of Dionysia Revoluta Boiss. Extracts on Acinetobacter Bumannii Isolated from Wound of Burned Patients.

Nahid Rahimifard¹, Leila Moslemi², Nooshin Aghilee³ and Mandana Moghni⁴

¹Department of Microbiology, Food and Drug Control Laboratories(FDCL), Minstry of health and medical Education (MOH), Tehran,Iran. Islamic Azad University, Pharmaceutical Sciences Branch, Faculty of Pharmacy, Tehran, Iran.

²Islamic Azad University, Pharmaceutical Sciences Branch, Faculty of Pharmacy, Tehran, Iran.

³Department of Food and Water Borne Disease. Tehran, Iran.

⁴Sharecord University of medical sciences, Iran.

Corresponding Authors E-mail: leila.moslemi1@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/2438

ABSTRACT: Dionysia revoluta Boiss  is a  member of primulaceae.This plant is distributed locally across south region of Iran. Antimicrobial activities against some species have been reported from the extracts and fractions of this plant. In the present study antimicrobial activities of the  methanol , chloroform and crude extracts against 50 isolates of Acinetobacter baumannii from wound of burned patients hospitalized at Motahari hospital in Tehran and Acinetobacter baumannii ATCC 10572 were evaluated. The samples of plant were collected from Hormozgan province. The plant were first dried and blended and extracts were prepared by standard methods of maceration. The extracts then were allowed to air dried. The dried concentrated extracts were kept within glass vials under standard conditions until used. 50 isolates of Acinetobacter baumannii from wound of burned patients were diagnosed by GNA-GNB microgen kit. Acinetobacter baumannii ATCC 10572 was used as standard strain. Standard agar diffusion methods (disk diffusion and well diffusion) were used to examine the antimicrobial effects of different concentrations of plant extracts against the bacteria. All three extracts had antibacterial effects on Acinetobacter baumannii. Water extract at 2000µg/ml showed the maximum inhibition zone (15 mm). Well diffusion methods was more efficient method and sixteen isolates (32%) were shown growth inhibition zone to all extracts and showed growth inhibition zone from 12 mm to 15 mm in this method. We concluded that the antimicrobial activity of Dionysia revoluta Boiss  against clinical isolates of Acinetobacter baumannii is valuable. Further investigation for determining of MIC and MBC and especially in vivo studies are recommended.

KEYWORDS: Acinetobacter baumannii; Antimicrobial burned patients; Dionysia revoluta Boiss;

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