Volume 12, number 2
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Optimization of Medium Components for Phenol Degradation by EM-1

S. Sivasubramanian*1, S. Karthick Raja Namasivayam2

1Department of Chemical Engineering, Faculty of Bio and Chemical Engineering, Sathyabama University, Chennai, Tamilnadu, India. 2Department of Biotechnology, Faculty of Bio and Chemical Engineering, Sathyabama University, Chennai, Tamilnadu, India.  

ABSTRACT: In this work phenol degradation was carried out using Effective Microorganism-1(EM-1) under optimized medium condition. The optimization of medium components was carried out by Plackett–Burman Design (PBD) and Central Composite Design (CCD).Screening of medium component by PB showed that initial phenol concentration(p=0.002), FeCl3(p= 0.002), MnSO4 H2O(p=0.001), MgSO4.7H2O (p=0.009) and KH2PO4   (p=0.004) had a significant effect on phenol degradation. The optimum of 96.8 % phenol degradation was observed at initial phenol concentration 1000 mg/l, FeCl3-1.5 mg/l, MnSO4 H2O-100mg/l,MgSO4.7H2O-100mg/ land KH2PO4-250mg/l.Second order polynomial regression analysis was carried out to study the interaction effects of medium composition. The R2==.9819 AdjR2= 0.99 and Pre R2= 0.981 suggested that experimental values are in good agreement with predicted values.

KEYWORDS: RSM; Plackett–Burman Design; Central Composite Design; Phenol degradation; Medium optimization

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Sivasubramanian S, Namasivayam S. K. R. Optimization of Medium Components for Phenol Degradation by EM-1. Biosci Biotech Res Asia 2015;12(2)

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