Combined Application of Different Species of Trichoderma and Pseudomonas Fluorescens on the Cellulolytic Enzymes of Fusarium Oxysporum for the Control of Fusarium Wilt Diseasei Arachis Hypogaea. L

P. Rajeswari¹ and Rupam Kapoor²

¹UGC Post- Doctoral Women Fellowship, Department of Botany, University of Delhi, India.

²Department of Botany, University of Delhi,110007 India.

Corresponding Author E-mail: aksharaasmitha @gmail.com

DOI : http://dx.doi.org/10.13005/bbra/2557

ABSTRACT: Fusarium oxysporum causes Fusarium wilt of crop plants leads to considerable yield loss. The study was conducted to determine the beneficial effects of combining Trichoderma species and Pseudomonas fluorescens i.e Trichodema viride+ Pseudomonas fluorescens (Tv+Pf) (1+2%), Trichoderma harzianum+Pseudomonas fluorescens (Th+Pf) (1.5+2%), Trichoderma viride +Trichoderma harzianum (Tv+Th) (1+1.5%) on the activity of cellulolytic enzymes of Fusarium oxysporum to control Fusarium wilt of Arachis hypogaea. L wilt in vitro. The activity of 1,4 -β – Endoglucanase, 1,4 -β – Exoglucanase, Cellobiases produced by Fusarium oxysporum (Control) was higher. Maximum inhibition of Cellulolytic enzymes was shown by culture filtrate of Trichoderma viride + Pseudomonas fluorescens (Tv+Pf) (1+2%), followed by Trichoderma harzianum + Pseudomonas fluorescens, (Th +Pf) (1.5+2%) and Trichoderma viride + Trichoderma harzianum (Tv+Th) (1+1.5%). However, disease suppression of Fusarium wilt of Arachis hypogaea. L by the compatible combination of Trichodema viride + Pseudomonas fluorescens (1+2%) was considerably better as compared to other two strains. At the same time the other two combinations resulted in enhanced disease suppression as compared to single strains.  This indicates that the potential benefits of using combination treatments to suppress Fusarium wilt. The study suggests the significance of interactive effects of Trichoderma and Pseudomonas in biocontrol of wilt disease.

KEYWORDS: Arachis hypogaea. Fusarium wilt; In vitro interactions; Trichoderma viride; Trichoderma harzianum; Pseudomonas fluorescens;  L

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