Induction of MPK3, MPK6 and MPK4 Mediated Defense Signaling in Response to Alternaria Blight in Transgenic Brassica Juncea

Mohd Tasleem^1,2, Mamta Baunthiyal¹ and Gohar Taj²

¹Department of Biotechnology, G. B. Pant Institute of Engineering and Technology, Pauri Garhwal, India.

²Department of Molecular Biology and Genetic Engineering, College of Basic Science and Humanities, G. B. Pant University of Agriculture and Technology, Pantnagar, India.

Corresponding Author E-mail: mohdtasleem99@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/2593

ABSTRACT: Alternaria brassicae causes a highly destructive disease in Brassica juncea (Rapeseed mustard) resulting in significant yield losses. Studies of MAPK machinery components in Arabidopsis thaliana have indicated that MPK3, MPK4, & MPK6 are involved in defense response and provide resistance against various bacterial and fungal pathogens. In this study, we analyzed the expression level of MPK3, MPK4 & MPK6 in overexpressed MPK3 transgenic (BjV5) Brassica juncea at different stages of Alternaria brassicae inoculation.Expression study revealed that MPK3/MPK6 was involved in early defense response and MPK4 in late defense response. These results suggested that BjMPK3 positively regulate SA mediated defense response, which might play an important role in resistance to Alternaria brassicae in Brassica juncea.

KEYWORDS:  Alternaria Brassicae; Brassica Juncea;MPK3; MPK4; MPK6 and BjV5

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