Volume 15, number 2
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Screening For Isolation of Succinic Acid Producing Microbes

Hema N and K. P. Sreenath

Department of Botany, Bangalore University, Bangalore - 560056, India.

Corresponding Author E-mail: hemamuniraj@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/2636

ABSTRACT: Succinic acid has emerged as one of the most competitive bio-based chemicals. Present study intended to isolate potential strains for the production of succinic acid. More than 90 isolates were obtained from various sources using anaerobic techniques. All the strains were screened for succinic acid production through bromocresol green media, thin layer chromatography and HPLC methods. 74 isolates showed colour change in bromocresol green media indicating the production of organic acids, of which 14 isolates showed succinic acid production determined by thin layer chromatography which were quantified through HPLC. The isolates which yielded  succinic acid ranged from 0.24g/l (MGC) to 4.69g/l (RFC-P3).

KEYWORDS: Anaerobes; Fluid Thioglycollate; Succinic Acid; HPLC

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Hema N, Sreenath K. P. Screening For Isolation of Succinic Acid Producing Microbes. Biosci Biotech Res Asia 2018;15(2).
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Hema N, Sreenath K. P. Screening For Isolation of Succinic Acid Producing Microbes. Biosci Biotech Res Asia 2018;15(2). Available from: https://www.biotech-asia.org/?p=29947

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