Study of Some Genetic Variants for Cancer in Women with Breast Cancer in the East Azarbaijan Region by MLPA Method

Mahdiyeh Pashaei¹, Jamal EivaziZiaei², Alireza Nikanfar³, Babak Emamalizadeh⁴ and Seyyed Mojtaba Mohaddes Ardebili⁵

¹Hematology and Medical Oncology, Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Department of Biochemistry and Clinical Laboratories, Division of Medical Genetics,Tabriz University of Medical Sciences, Tabriz, Iran.

²Hematology and Medical Oncology, Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Iran.

³Tabriz University Hematology and Oncology Research Center, Shahid Ghazi Hematology and Oncology Ward.

⁴Department of Medical Genetics, Tabriz University of Medical Sciences, Tabriz, Medical Genetics.

⁵Department of Biochemistry and Clinical Laboratories, Division of Medical Genetics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.

Corresponding Author E-mail: mojtabamohaddesardebili197@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/2675

ABSTRACT: Breast cancer is one of the main factors in the mortality of Iranian women. A large rearrangement genome is observed in most genes, especially in BRCA1 / BRCA2 genes lacking small mutations in breast cancer. Therefore, methods are needed to detect one or more exon deletions or their duplication. Therefore, the aim of this study was to determine the change in the number of copies of ATM, BRCA1, CHEK2, PTEN, and P53 genes in women with breast cancer in the East Azarbaijan region by MLPA method. This research is a descriptive study that was conducted randomly among 150 Azeri women with breast cancer who were referred to Tabriz Nour Najat Hospital; sixteen healthy people were selected as control samples.  Deletion and duplication of ATM, BRCA1, P53, CHEK2 and PTEN genes were investigated using the MLPA method. The results showed that there was no pathogenicity mutation in these five genes. Therefore, it can be said that a large rearrangement genome in the East Azarbaijan province is very unlikely to lead to breast cancer in the area.

KEYWORDS: ATM; BRCA1;  Breast Cancer; CHEK2; MLPA; P53 PTEN;

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