Effect of Vitamins on Growth and Shoot Multiplication of Chlorophytum borivilianum

K. Menaria¹*, S. D. Purohit¹, R. P. Rajpu² and R. K. Tenguria³

¹Department of Biotechnology, M.L. Sukhadia University, Udaipur (India). ²JNKVV, Jabalpur (India). ³Division of Microbiology, Department of Botany, Govt. MVM, Bhopal (India).

ABSTRACT: The nutrient media play an important role in plant tissue culture as it affects the growth and multiplication of the plant. The various standard media i.e. MS and White Medium are being used for plant tissue culture. The nutrient requirement of the plant varies with plant species. The effect of various vitamins (Biotin, Riboflavin, Ca- pantothenate, Folic acid and Ascorbic acid) with different concentrations (0,1.0, 2.5,5.0ppm), were studied for growth and multiplication of Chlorophytum borivilianum. Among five different vitamins the Biotin @ 2.5 ppm proved to be the best vitamin additive with MS medium for growth and multiplication of Chlorophytum borivilianum.

KEYWORDS: Vitamins; shoot multiplication; Chlorophytum borivilianum

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