Antifungal Effect of Flavonoid Extract of Trachyspermum Ammi Plant on the Gene Expression of Pro-inflammatory Cytokines such as IL-18 and TNF-α in Articular Chondrocyte Cells

Maryam Sadat Abtahi¹ , Zinat Mohammadi², Boshra Hatef³*, Masoomeh Amini⁴, Mehdi khorrami⁴ and Mohammad-Reza Aghanoori⁴

¹Department of Department of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran ²Department of Plant Physiology, Science and Research Branch Islamic Azad University, Tehran, Iran. ³Professor Assistant of Neuroscience Research Center of Baqiyatallah University of Medical Science, West Artesh Highway, Tehran, Iran. ⁴Department of Medical Genetics, Shiraz University of Medical Scienes, Shiraz, Iran.

DOI : http://dx.doi.org/10.13005/bbra/1876

ABSTRACT: The Trachyspermum ammi plant has anti-fungal properties. Some fungi such as a yeast two Sporothrix schenchii produces the arthritis in pack animals. The aim of this study is to examine the anti-fungal effects of this plant on the pro-inflammatory bio-markers, which are important in the course of fungal diseases. At first, the ethanol extract of the Trachyspermum ammi plant was provide by the Iranian biological resource center. We used chondrocyte cells as samples because they were similar to the cells with fungal infection. Then chondrocytes (5×105 cells/well) were incubated at a humidity of 90%, 5% CO2 and 37ÚC for 72 h with control media alone or with Trachyspermum ammi at concentrations of 10 ìg/ml. One set of cells was activated for 1 h with mannose (3 mg/ ml) for both reverse-transcriptase PCR and real-time PCR analysis of Tumor necrosis factor alpha (TNF-á) and Interleukin-18 (IL-18) expression. The activated cells by mannose treated by the Trachyspermum ammi extract reduced TNF-á expression as activated cells by mannose with fluconazole. However the IL-18 gene expression was reduced by both treatments but fluconazole had better effect. Based on the PCR and Real-time PCR methods analysis of inflammatory gene expression, Trachyspermum ammi plant such as the flavonoids has noticeable anti-fungal properties on chondrocyte cells.

KEYWORDS: Trachyspermum ammi; fluconazole; chondrocyte cells; Anti-fungal Infection

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