Influence Of Sucrose Concentration On Growth And Ajmalicine Production From Catharanthus Roseus Suspension Cultures

A. G. Namdeo¹*, D. P. Fulzele², S. Patil³ and S.S. Kadam¹

¹Department of Pharmacognosy and Phyto-Biotechnology, Poona College of Pharmacy, BVDU, Erandwane Educational Campus, Pune (India). ² Biotechnology Division, Bhabha Atomic Research Centre, Mumbai (India). ³ School of Life Sciences, DAVV, Indore (India)

ABSTRACT: Sucrose is a major ingredient of culture medium. Present study was performed to investigate the influence of sucrose concentration on growth and ajmalicine production. Maximum packed cell volume (72%), fresh weight (65g 100 ml-1), and dry weight (6.2148g 100 ml-1) was recorded in medium containing 4% sucrose. About 9-fold increase in dry weight was recorded in medium with 4% sucrose. All growth parameters increased upto 25th day of incubation after which a gradual decline was observed. Ajmalicine accumulation started from 15th day of incubation and reached to maximum (60 μg g-1 dry weight) in 25th day of incubation in medium with 4% sucrose, which however gradually reduced on further incubation.

KEYWORDS: Catharanthus roseus; sucrose; ajmalicine; suspension culture

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