Volume 4, number 2
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Argentated silica gel chromatography for separation of g-linolenic acid from microalgae

Srinivasa Reddy Ronda1* and Smita Lele2

1Koneru Lakshmaiah College of Engineering, Vaddeswaram, G?nter - 522 502 (India) 2University Institute of Chemical Technology, Matunga, Mumbai - 400 019 (India)

ABSTRACT: A low expense process is developed for recovery of g-linolenic acid from Spirulina platensis biomass. Simultaneous extraction and direct transmethylation of fatty acids from freeze dried biomass and subsequent purification using silver silica gel column chromatography resulted in 95.43% pure g-linolenic acid. The percent purity of g-linolenic acid in the fractions varied from 87.18 -95 %. A recovery of 74.05% g- linolenic acid was achieved using silver silica gel chromatography.

KEYWORDS: g-linolenic acid; silver silica gel column; chromatography; methyl ester

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Ronda S. R, Lele S. Argentated silica gel chromatography for separation of g-linolenic acid from microalgae. Biosci Biotechnol Res Asia 2007;4(2)
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Ronda S. R, Lele S. Argentated silica gel chromatography for separation of g-linolenic acid from microalgae. Biosci Biotechnol Res Asia 2007;4(2). Available from: https://www.biotech-asia.org/?p=6026

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