Volume 5, number 1
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Development and validation of high performance thin layer chromatographic (hptlc) technique for quantification of glipizide in tablet dosage forms

Shelesh Jain and Swarnlata Saraf*

Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur - 492 010 India

ABSTRACT: A new simple, accurate and precise HPTLC method has been developed for the estimation of glipizide in tablet formulation. In this method, standard and sample solutions of glipizide were applied on precoated silica gel G 60 F254 TLC plate, and developed using ethyl acetate: formic acid: dichloromethane (1:1:2 v/v/v), as mobile phase. The drugs on plate were scanned at 275.5 nm. The dynamic linearity range was 200-800 ng/spot for glipizide. The method was validated for linearity, accuracy, limit of detection, limit of quantification, inter-day and intra-day assay precision, repeatability of measurement, and repeatability of sample application.

KEYWORDS: HPTLC; Glipizide; tablet dosage forms

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Jain S, Saraf S. Development and validation of high performance thin layer chromatographic (hptlc) technique for quantification of glipizide in tablet dosage forms. Biosci Biotechnol Res Asia 2008;5(1)
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Jain S, Saraf S. Development and validation of high performance thin layer chromatographic (hptlc) technique for quantification of glipizide in tablet dosage forms. Biosci Biotechnol Res Asia 2008;5(1). Available from: https://www.biotech-asia.org/?p=6940

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