Antibacterial Sensitivity Kinetics of Urinary Staphylococcal Isolates in Benin City; A Case for Synergy?

O. A. Oni¹, T. C. Ozoko², Otuaga³, M. Aghimien⁴ and P. Onyesom⁵

¹Consultant Physician,Department of Internal Medicine College of Health Sciences, Niger Delta University Wilberforce Island, Amassoma, Bayelsa state Nigeria.

²Department of Medical Microbiology and Parasitology College of Health Sciences, Delta State University, Abraka, Nigeria.

³Department of Anatomy, College of Health Sciences Delta State University, Abraka

⁴Department of microbiology, Igbinedion University, Okada Edo State, Nigeria.

⁵Department of medical Biochemistry College of Health Sciences, Delta State university, Abraka, Nigeria.

Corresponding Author E-mail: onisare@yahoo.com

ABSTRACT: Two hundred and fifty isolates of Staphylococci were obtained from urine samples of patients presenting with urinary symptoms at University of Benin teaching Hospital microbiology laboratory. The isolates were identified and the 180 isolates of Staphylococcus aureus were classified on the basis of b-lactamase production and subjected to dilution antibacterial sensitivity tests and minimum inhibitory concentration (MIC) determination using ampicillin and gentamicin. As expected ampicillin was active against b-lactamase negative organisms but was resisted by the positive organism however there was significant reduction in bacterial count at high doses. Gentamicin showed activity with MIC at 5 μg/ml for the negative organism and 10μg for positive organisms. The above finding support the growing recognition of Staphylococcus aureus as a urinary pathogen and suggest the possibility of cheap effective chemotherapy by synergistic combination of ampicillin and gentamicin in management of Urinary infection, especially where Staphylococcus aureus is expected.

KEYWORDS:

b-lactamase; minimum inhibitory concentration (MIC); Synergism; Malaria; Quinine

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