Evans Blue Extravasation to Brain by Surfactant Modified Liposomes

Muthuprasanna¹*, Manisha Mishra²  and K. Suriaprabha¹

¹Department of pharmaceutics, Hindu College of Pharmacy, Guntur India.

²CARISM, SASTRA, Tanjavur India.

Corresponding Author E-mail: p_ra2000@yahoo.com

ABSTRACT: Liposome are colloidal, vesicular structures capable of targeting to specific sites. Evans blue dye ( hydrophilic dye) were formulated in liposomes using phosphatidyl choline, cholesterol and those liposomes were modified using surfactants surfactants span 20 , span 40 , span 80 or tween 80 at various molar ratio and optimized for its stable vesicular formation. The particle size and FTIR analysis were performed for those formulations. Invivo studies were performed using those surfactant modified liposomes containing a biological marker - Evans blue dye to study the formulation penetrating power to brain. Span 80 and tween 80 combination of molar ratio 2:1 was efficient in modifying the liposomes towards delivering the dye to brain bypassing blood brain barrier than other surfactant modification of liposomes and the liposomes without surfactant. The result was further substantiated by carrying out SDS-PAGE gel electrophoresis using the brain homogenates, which showed visible bands of Evans blue dye for Span 80 tween 80 combinations indicating the targeting potential of surfactant modified liposomes to the brain. These results suggests that surfactants Span 80 tween 80 combination (molar ratio 2:1) used to modify liposomes proved to be the best carrier for desired drug to penetrate Blood brain barrier .

KEYWORDS: Liposome; Span 80; Tween 80; PAGE Gel Electrophoresis; Evans blue

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