Volume 5, number 2
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Purification and Identification of 70 Kda Heat Stable Protein

Aara Rifat, S. A. Hajam and K.I. Andrabi

Department of Biochemistry, University of Kashmir, Kashmir - 190 006 India.

ABSTRACT: A heat stable protein (hsp) has been purified to homogeneity from sheep liver extract by heating to 95 O C followed by ion-exchange chromatography on DEAE-cellulose (DE-52). The purified protein showed a single band when examined by gel electrophoresis. The molecular weight determined by SDSpage was found to be approximately 70KDa. The presence of this protein was also demonstrated in whole cell homogenate, heat stable and nuclear fraction.

KEYWORDS: Hsp; SDS-page

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Rifat A, Hajam S. A, Andrabi K.I. Purification and Identification of 70 Kda Heat Stable Protein. Biosci Biotechnol Res Asia 2008;5(2).
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Rifat A, Hajam S. A, Andrabi K.I. Purification and Identification of 70 Kda Heat Stable Protein. Biosci Biotechnol Res Asia 2008;5(2). Available from:

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