Minimum Inhibitory Concentration and Time Kill Curve Studies of Tobracef, A Fixed Dose Combination of Ceftazidime and Tobramycin

Sanjay Mohan Shrivastava*, Sanjeev Kumar Shukla and Manu Chaudhary  

Venus Medicine Research Center, Hill Top Industrial Estate, Jharmajri EPIP, Phase I (Extn) Bhatoli Kalan, Baddi India.

Corresponding Author E-mail: dgmtechnical@venusremedies.com

ABSTRACT: Ceftazidime belongs to cephalosporin class of antibiotics with broad spectrum activity. Tobramycin is an aminoglycoside antibiotic used to treat various types of bacterial infections, particularly gram negative infections. This study was aimed at evaluating microbial efficacy of Tobracef, a Fixed Dose Combination (FDC) of ceftazidime and tobramycin in comparison with ceftazidime and tobramycin alone. Efficacy was evaluated on the basis of Minimum Inhibitory Concentration (MIC) and time kill curve analysis in Acinetobacter baumanii, Mycobacterium smegmatis, Citrobacter braaki and Pseudomonas aeruginosa. In case of A. baumanii, M. smegmatis, C. braaki and P. aeruginosa, MIC were found to be 0.0625 mg/l, 0.125 mg/l, 0.03125mg/l and 0.25 mg/l in Tobracef respectively. In ceftazidime alone the MIC were found to be 0.25mg/l, 0.25mg/l, 1mg/l and 1mg/l respectively. For tobramycin alone the MIC were found to be 0.125mg/l, 0.25mg/l, 0.0625mg/l and 0.5mg/l respectively. In all organisms under study, time-kill curve analysis demonstrated bacterial maximum killing at 4 hours. In conclusion, Tobracef, a FDC of ceftazidime and tobramycin was found to have more bacterial inhibiting properties than ceftazidime and tobramycin alone.

KEYWORDS: Minimum Inhibitory Concentration (MIC); ceftazidime; tobramycin; tobracef

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