Volume 7, number 2
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Production of Alpha Amylase Using Bacillus Subtilis Ncim No. 2724 under Solid State Fermentation

B. Manuel Choragudi1*, B. Ranjani Choragudi1, S. Felice Choragudi2 and V. Saradhi Settaluri2

1Sujay Bio-Tech Pvt. Ltd., Plot No.19A, Phase - III, 3rd Road, Jawahar Autonagar, Vijayawada India.

2Koneru Lakshmaiah College of Engineering, Green Fields, Vaddeswaram India.

Corresponding Author E-mail:  ben_cmanuel@rediffmail.com

ABSTRACT: Production of alkaline amylase by Bacillus sp. under solid state fermentation (SSF) was optimized. The effect of wheat bran and rice bran were examined. The appropriate Incubation time, Temperature, pH, Inoculum size, Moisture content, Carbon source, Nitrogen source, Substrate concentration, Surfactant and Mutagenic effects were determined. Maximum yield of enzyme was achieved by employing wheat bran as substrate ((94±2) U/g) at 72hrs with 1:1.5 moisture content and pH-8. Inoculum size was found to be 30% for wheat bran. The increased growth of bacterium of mutagenic activity is seen with acrylamide solution.

KEYWORDS: Enzymes; alfa-amylase; wheat bran; rice bran

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Choragudi B. M, Choragudi B. R, Choragudi S. F, Settaluri V. S. Production of Alpha Amylase Using Bacillus Subtilis Ncim No. 2724 under Solid State Fermentation. Biosci Biotech Res Asia 2010;7(2)
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Choragudi B. M, Choragudi B. R, Choragudi S. F, Settaluri V. S. Production of Alpha Amylase Using Bacillus Subtilis Ncim No. 2724 under Solid State Fermentation. Biosci Biotech Res Asia 2010;7(2).Available from:https://www.biotech-asia.org/?p=9396

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