Volume 7, number 1
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A Year-Round Aero Mycological Study by Culture Plate Technique at Government J. P. Verma P.G. Arts And Commerce College, Bilaspur (C.G.)

A. Sonia1, A. Kabir1, U. Singh2 and N. K. Singh3

1*Department of Microbiology & Botany, Government J. P. Verma P.G. Arts & Commerce College, Bilaspur India. 2Department of Botany, Govt. Bilasa Girls P. G. College, Bilaspur India. 3Department of Botany, Govt. Science P. G. College, Bilaspur India. COrresponding Author E-mail:asonia15@gmail.com

ABSTRACT: The study of aerobiology has acquired a prominent place in various fields of environmental sciences. Environmental factors are the most important physical factors which affect the fungal population in the air. The present paper deals with the aero mycological studies at Govt. P. G. J. P. Verma Arts and Commerce College, Bilaspur from Feb. 2008 to Jan. 2009. For the survey of aeromycoflora, the method used for the isolation was culture plate method. This involves the principal of sedimentation under the force of gravity. Petri plates containing PDA were exposed for five to ten minutes in air at fortnightly interval from sampling site, the plates were then incubated at 370C for 3-4 days. After incubation period, isolated fungal colonies were counted and identified with the help of available literature. Altogether 12 different fungal species were obtained which were purified by single colony isolation method. Amongst these Aspergillus niger,A. flavus, A. nidulans, Penicillium species were dominant followed by species of Rhizopus, Trichoderma etc. Fungal spores showed time trends during the study period, as it was found that lowest number of fungi were isolated in summer season. On the other hand, fungal isolates showed a greater degree of prevalence in winter followed by rainy season. The study also revealed that Aspergilli were the common fungi present in the air of the sampling site. Identification of remaining species and physiological characterization is in progress.

KEYWORDS: Aeromycoflora; Culture Plate method; Percentage frequency; sedimentation.

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Sonia A, Kabir A, Singh U, Singh N. K, A Year-Round Aero Mycological Study by Culture Plate Technique at Government J. P. Verma P.G. Arts And Commerce College, Bilaspur (C.G.). Biosci Biotech Res Asia 2010;7(1)

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Sonia A, Kabir A, Singh U, Singh N. K, A Year-Round Aero Mycological Study by Culture Plate Technique at Government J. P. Verma P.G. Arts And Commerce College, Bilaspur (C.G.). Biosci Biotech Res Asia 2010;7(1). Available from: https://www.biotech-asia.org/?p=9778

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