Study on Effect of Pectin Based Edible Coating on the Shelf Life of Sapota Fruits

Edible coating is defined as the thin layer of edible components formed on the surface of food materials. It has the similar effects of modified atmospheric packaging which alters the atmosphere surrounding the food products. It is used as a post-harvest technique to retain and improve the quality of the food products such as fruits and vegetables. In the present work the edible coating was prepared using the polysaccharide pectin (3%), glycerol (2.5%), polyvinyl alcohol (1.25%) and citric acid (1%) and the sapota fruits were coated using the dipping method. The both control and coated fruits were stored at room temperature (30±3oC). The physicochemical parameters were measured in the regular interval of time i.e., 1, 3, 5, 7 and 11th day of the storage. The pectin coated sapota fruits showed reduced rate of change in weight loss, acidity, TSS, pH, colour, ascorbic acid and the firmness when compared to that of control fruits. The shelf life of coated sapota fruits were extended upto 11 days where as the control fruits were in the edible state upto 6th day at room temperature storage.

The marketability of the food products such as fresh fruits and vegetables mainly depends upon appearance, flavor, colour, texture and nutritional value and microbial safety.The maintenance of these properties is a challenging task [1] .Different techniques have been used in the preservation of these food products; one such technique is edible packaging including edible coatings and films.The edible films and coatings are the primary packaging which is prepared from edible materials [2] .These are used as the preservation technique of agricultural produce and many other food products because of their barrier properties against the transfer of gas, moisture and solute molecules between the food and the surrounding.These coatings and films can be consumed along with the food product which is considered as their main advantage over the synthetic packaging [3] .The concept of application of edible coatings on the agricultural produce is not new.It has been found that in the early 12 th century, the fruits like oranges and lemons were coated with wax to retain their quality and extend the shelf life for longer period of time [4] .Since then these edible films and coatings have been used on different fruits in order to extend their shelf life.
Sapota (Achras sapota Linn.) is a perishable fruit which has played major role in the traditional Indian medicine because of its various useful properties [3] .India is considered to be the largest producer of sapota fruits with annual production of 11.17 lakh tones, followed by the countries Mexico, Guatemala and Venezuela.Sapota fruits are the rich source of sugar containing 12 to 14% [5] .It is also a source of chicle gum which is used in the manufacture of chewing gum [6] .In addition to these, sapota fruits also contain acids, amino acids, proteins, minerals such as potassium, iron, calcium; phenolic compounds such as catechin, carotenoids, gallic acids, chlorogenic acid, ascorbic acid, leucodelphinidin, leucopelargonidin and leucocyanidin [3] .The different food products such as jam, osodehydrated slices, jellies, squash, dries sapota pieces, milk shake, blended sapota drinks, nectar, sweet chutney, pickle, candy and preserve can be produced from the sapota fruits [5] .Sapota fruits are highly perishable and there is requirement for the effective preservative method to improve their post harvest life.The edible packaging including edible films and coatings can be used to extend the shelf life of these sapota fruits.
Edible coatings and films are made up of three different components; they are polysaccharides (such as cellulose and its derivatives, pectin, starch, dextrins, alginates and plant gums), proteins (such as soy proteins, milk proteins, collagen, corn zein and albumins) and lipids (such as fatty acids and their esters, monodi -and triglycerides, beeswax, carnauba etc.) [3][7] .These components have their own advantages and disadvantages and their effect on the shelf life extension of fruits depends on the storage conditions [8] .Chitosan, pectin derivatives, cellulose, starch, seaweed extracts etc are the most commonly used polysaccharide components in the preparation of edible coating.These polysaccharide based coatings have better mechanical strength but are poor in terms of moisture and gas barrier properties [9] .
Pectin is a polysaccharide made up of galactouronic acids and these are considered as amorphous, white coloured colloidal carbohydrate.The fruits such as apples, currants contain the high concentration of pectin in their ripened stage [10] .In the food industries the pectin is used as gelling and stabilizing agents [11] .These have been used in the preservation of many fruits and vegetable such as guava, apples, avocado, carrot, papaya, tomato etc [10] .The strawberries coated with pectin based edible coating showed reduced rate of change in the firmness, weight loss, and also reduced the spoilage of fruits due to microbial infection [12] .The combination of pectin coating and the osmotic dehydration has been used in the preservation of fresh cut melons in the refrigerated temperature storage [13] .The pectin based edible coating incorporated with oregano essential oil was found to be effective in reducing the fungal decay and also resulted in the increased antioxidant activity of tomatoes [14] .The effect of the pectin coating containing green tea extracts was studied on the quality of irradiated pork patty [15] .

The preparation of pectin based edible coating solution
The pectin (3%) is dissolved in distilled water at the temperature of 90 0 C. To this pectin solution, glycerol (2.5%), polyvinyl alcohol (1.25%) and citric acid (1%) is added and mixed using the magnetic stirrer.After mixing, the solution is cooled to room temperature and allowed to degas [12] .

Physico chemical analysis of fruits Weight loss
The weight loss in control and the coated fruit samples is measured using the following formula [16] .

Total soluble solids (TSS)
The TSS content of the fruits is determined using the hand refractrometer.A drop of fruit juice is placed onto the plate surface of the refractrometer and the reading is taken directly as º Brix [11] .pH The pH of the fruit pulp is measured using the digital pH meter.Initially the pH meter is calibrated using the pH 7 buffer solution and then the probe of the pH meter is placed into the fruit juice and reading is taken directly [17] .

Titratable acidity
Acidity is measured by the titration method using 0.1 N NaOH solution.The acidity is measured using the following formula [17].

Colour
The L * , a * and b * values of the fruit peel is measured during the storage using the Hunter Colourimeter.The L * values depicts the lightness, a * values represent the extent of the redness or greenness and b * values represent the extent of blueness or yellowness [17] .

Ascorbic acid
Ascorbic acid is the acid which is present in the highest amount in the sapota fruits.It is measured by the titration method using 2, 6 dichlorophenol indophenol [17] .
Ascorbic acid mg 100g of sample = 0.5 mg * V1 * 100 ml V1 * 5 ml * weight of sample * 100 Where, V 1 is the titre value of standard ascorbic acid V 2 is the titre value of fruit pulp.

Firmness
The firmness of both control and the coated sapota fruits was measured during the storage period by using the texture analyzer.The penetration test was used to determine the firmness of fruits.A probe of 2 mm diameter was allowed to penetrate through the fruits upto 10 mm depth.During the analysis, the load cell of 50Kg, pretest speed 1.5mm/sec, test speed 1.0mm/sec, post test speed 10mm/sec was maintained.The force that is required to penetrate through the surface of fruits was measured in terms of gram [11] .

RESULTS AND DISCUSSION
The coated and uncoated sapota fruits were stored at room temperature (30±3 º C).The physico chemical parameters such as weight loss, TSS, titratable acidity, pH, colour, ascorbic acid and firmness of both control and coated sapota fruits were measured during the storage.

Weight loss
The weight loss of control fruits increased from 2.22±0.5% to 16.98±0.4%whereas the weight loss in the coated fruits increased from 1.19±0.1% to 17.28±0.5% as shown in table 1.It was found that, the sapota fruits coated with 2.5% corn starch showed the weight loss of 17.56% on 9 th day of storage [18] .pH The pH value of both coated and control sapota fruits decreased during the storage.The pH value of control fruits ranged from 5.27±0.05 to 5.96±0.03and that of coated fruits ranged from 5.34±0.01 to 5.92±0.04 as shown in table 1.

TSS
The value of TSS increased as the fruits ripened.In the case of control fruits, TSS value increased from 21±0.00 º Brix to29±0.00º Brix where as in the coated fruits the TSS value increased from 22±0.00 º Brix to 31±0.00 º Brix as shown in table 2. In the corn starch (2.5%) coated sapota fruits TSS was found to be 28.19 º Brix on 9 th day of storage [18] .

Titratable acidity
The titratable or total acidity of control fruits decreased from0.405±0.6% to 0.16±0.09%where as in the coated fruits acidity decreased from 0.426±0.1% to 0.18±0.2%as shown in table 2. The sapota fruits coated with 2.5% corn starch showed the acidity of 0.45% on 3 rd day of storage at ambient conditions [18] .

Ascorbic acid
The ascorbic acid content of control fruits reduced from 14.68±0.5mg/100g of fruit pulp to 6.08±0.3mg/ 100g of fruit pulp where as in the coated fruits the ascorbic acid decreased from 14.2±0.13mg/ 100g of fruit pulp to 5.91±0.15mg/100g of fruit pulp as shown in table 3. It was reported that, in the sapota fruits coated by corn starch (2.5%), the ascorbic acid content was decreased to 9.51% on the 9 th day of storage [18] .

Firmnes
The firmness of both control and coated sapota fruits reduced during the storage period.This reduction was at the higher rate in the control fruits when compared to that of pectin coated fruits.The flesh firmness of control and coated fruits measured on 1,3,5,7 and 11 th day of storage and it ranged from 429.49±0.3 to 79.87±0.45gand from 563.49±0.42 to 75.337±0.6grespectively as shown in table 3.

Colour measurement
L * , a * , b * values of sapota peel were measured.L * value of peel decreased as the fruits ripened.In the case of control fruits, L * value of peel decreased from 48.2±0.61 to 39.47±0.11and in the coated fruits, the L * value decreased from 49.27±0.53 to 39.32±0.1 as shown in table 4.
The a* values of peel of both control and coated fruits increased during the storage.The a* value of the peel of the control fruits increased from 5.77±0.4 to 11.52±0.34and that of coated fruits increased from 5.24±0.9 to 11.57±0.63during the storage as shown in table 3.As the fruits ripened during the storage, the b * value of peel of both control and coated fruits decreased.In the case of control fruits, the b * value of peel decreased from 28.62±0.5 to 15.44±0.31and that of coated fruits decreased from 29.65±0.26 to 16.49±0.64as shown in table 4.

CONCLUSION
The pectin based edible coating extended the shelf life of sapota fruits upto 11 days by delaying the changes in the physico chemical parameters such as weight loss, TSS, pH, total acidity, ascorbic acid, firmness and colour where as the uncoated sapota fruits were in the edible state upto 5 th day at room temperature (30±3 º C).
Acidity(%) = Titre value * normality of NaOH * total volume * acid factor weight of the sample * volume of the filtrate taken * 100

Table 1 .
Weight loss and pHvalues of control and coated sapota fruits

Table 2 .
Acidity and TSS values of control and coated sapota fruits

Table 3 .
Ascorbic acid and firmness values of control and coated sapota fruits

Table 4 .
L * , a * and b * values of control and coated sapota peel