Optimization of Parameters for Decolorization of Reactive Dyes using Bacterial Isolates

K. Suganya¹, K. Revathi^1*, V. Anuradha² and K. Gopi³

¹Department of Zoology, Ethiraj College for Women, Chennai-8, Tamilnadu, India. ²Department of Biochemistry, Mohamed Sathak College of Arts and Science, Chennai - 119, India. ³Centre for Marine Bioprospecting AMET University, Kanathur, Chennai - 603112, India

DOI : http://dx.doi.org/10.13005/bbra/1428

ABSTRACT: The present study was carried out to degrade the textile Reactive azo dyes by using bacteria isolated from dye contaminated soil. The effect of pH, temperature, carbon, nitrogen souces, inoculum size and incubation time was studied with an aim to determine the optimal conditions required for maximum decolorization. The bacterial strains used in the study were Bacillus licheniformis and Pseudomonas putida. The optimum pH for decolorization of RR 195 by P. putida was 7.0. It shows good decolorization efficiency even in alkaline region. The optimum temperature was 370C. Glucose and Yeast extract has emerged as the ideal carbon and nitrogen sources for both bacteria. In different inoculum size and incubation time evaluated, 3.0% v/v and 120 hrs appeared to highest percentage of decolorization process by both bacterial strains.

KEYWORDS: Decolourization; Reactive Azo Dyes; Optimization; Bacillus licheniformis; Pseudomonas putida.

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