In vitro propagation of Chlorophytum borivilianum (Safed musli) through mature explant

S. A. Hajaam², M. FARHEEN³, A. A. Naik¹*, A. Wanganeo¹ and I. A. Sheikh²

¹Department of Limnology, Barkatullah University, Bhopal - 462 026 (India) ²Department of Biosciences Barkatullah University, Bhopal - 462 026 (India) ³Government MVM College, Bhopal - 462 001 (India)

ABSTRACT: Chlorophytum borivilianum, commonly known as safed musli is an endangered medicinal herb valued for its dried, fasciculated storage roots which possess immunomodulatory and aphrodisiac properties that forms an important ingredient of herbal tonics. Efficient micropropagation of C. borivilianum employing liquid medium has been achieved. In the case of employing liquid medium, while on the one hand lesser amount of medium is consumed, on the other hand shoot growth and multiplication response were better than in solid medium. The basic nutrient medium (MS) Murashige and Skoog (1962) supplemented with different phytohormone concentrations viz. cytokinins “BAP (0.5-5mg/l), kinetin (0.1-0.5mg/l), alone and with combination of auxins (NAA 0.1-1mg/l) with 3% sucrose and 4.5 gm/l agar” were used. The pH of the medium was adjusted between 5.5-5.8 by using 0.1 N HCl or 0.1 N NaOH before sterilization. The cultures were maintained at a temperature range of 25±2 OC and the photoperiod of 16 hrs by using white fluorescent light (2000-3000 Lux).

KEYWORDS: Chlorophytum borivilianum; growth regulators; tissue culture

This work is licensed under a Creative Commons Attribution 4.0 International License.