Determination of Trace Amounts of Methamphetamine in Biological Samples by Hollow Fiber Liquid-phase Microextraction Followed by High Performance Liquid Chromatography

Mina Darvish¹, Mahnaz Qomi¹*, Maryam Akhgari², Parviz Raoufi²

¹Department of Medicinal Chemistry (Pharmaceutical Sciences Research Center) Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran. ²Legal Medicine Research Center, Legal medicine organization, Forensic Toxicology Department , Tehran, Iran.

ABSTRACT: Methamphetamineis used to treat Attention-deficit hyperactivity disorder (ADHD) or obesity as Desoxyn. In this work, for the first time a microextraction technique was introduced to detection and measurement of methamphetamine in urine and plasma samples. Hollow ûber based liquid phase microextraction (HF-LPME) followed by high performance liquid chromatography (HPLC) coupled with ultraviolet (UV) detection was used for extraction of methamphetamine. The organic solvent consists of 1-Octanol immobilized in the pores of a hollow fiber wall. A pH gradient was applied to migrate analytes from the sample solution with pH 11.6, through the organic solvent membraneinto an acidic acceptor solution with pH 2.8which was located inside the lumen of hollow fiber. Extraction recoveries upper than90% were obtainedin different biological matrices which resulted in preconcentration factors upper than 131.5 and acceptable repeatability (3.4< RSD%(n=6)<3.9). The method offers good linearity with estimation of coefficient higher than 0.9850. Finally, it was applied tothe detection and determination of methamphetamine in human plasma and urine samples.

KEYWORDS: Methamphetamine; High performance liquid chromatography; Hollow ûber based liquid phase microextraction; Microextraction

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