Micropropagation of Orchid Dendrobium barbatum Lindl. (Orchidaceae) from Fleshy Stem Segments

M.N. Abubacker^1*, J. Durgadevi² , C. Sathya³ and K. Leema Rose⁴

1-2Department of Biotechnology, National College, Tiruchirappalli - 620 001, India. 3 Department of Botany , Alagappa University, Karaikudi - 630 003, India. 4Department of Botany, National College, Tiruchirappalli - 620 001, India.

DOI : http://dx.doi.org/10.13005/bbra/1145

ABSTRACT: An efficient protocol for micropropagation of orchid Dendrobium barbatum Lindl. (Orchidaceae) using the fleshy stem segments was developed. Murashige Skoog (MS) basal medium supplemented with 1.0 mg L of Naphthalene Acetic Acid (NAA) + 2 .0mg L of Benzyl Amino Purine (BAP) for multiple shoot induction. The multiplied shoots were then transferred to half-strength MS medium supplemented with 1 mg L of Indole-3 acetic acid (IAA) or Indole-3 butyric acid (IBA) or NAA. Best response for shoot multiplication was achieved when the medium was supplemented with 1 mg L-1 NAA and 2mg L-1 BAP. While that for rooting with 1 mg l IBA. The plantlets were hardened off and survived plants were released to the shade house then to their epiphytic environment.

KEYWORDS: Benzyl Amino Purine (BAP); Dendrobium barbatum fleshy stem segment; In vitro

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