Gas Chromatography - Mass Spectroscopy (GC-MS) Analysis of Various Extracts of Cocculus hirsutus
B. Samuel Thavamani1*, Molly Mathew2 and S. P. Dhanabal3
1Deptartment of Pharmacognosy, PSG College of Pharmacy, Coimbatore, Tamilnadu, India, 2Department of Pharmacognosy, Malik Deenar College of Pharmacy, Kasargode, Kerala, India. 3Department of Phytopharmacy and Phytomedicine, JSS College of Pharmacy, Ooty, India.
ABSTRACT: Gas Chromatography - Mass Spectroscopy (GC-MS) analysis was used to study the bioactive compounds present in the petroleum ether, chloroform and methanol extracts of Cocculus hirsutus. GC-MS analysis of petroleum ether extracts showed the presence of 5 compounds of which 1 is a nitrogenous compound. Chlorofom extract of Cocculus hirsutus showed the presence of 15 compounds of which 11 are nitrogenous compounds. In the same way methanol extract of Cocculus hirsutus showed the presence of 12 compounds of which 8 are nitrogenous in nature. This GC-MS analysis gives an idea that phytoconstituents present in Cocculus hirsutus are rich in nitrogenous substances. Some of the nitrogenous compounds identified from this plant in this study are Aziridine, 2-hexyl-, Azocine octahydro-, Boraneamine-N-ethyl-1,1-dipropyl-, 4-[1,4]Diazepan-1-yl-1,5-dihydro-imidazol-2-one, Pyrazol-3(2H)-one,4-(5-hydroxymethylfurfur-ylidenamino) -1,5-dimethyl-2-phenyl-, Cinnamic acid, a-[N-benzoylamino]-3,5-di-t-butyl-4-hydroxy- with the peak area of 2.12%, 3.04%, 2.64%, 2.38%, 12.75% and 33.04.
KEYWORDS: GC-MS; Cocculus hirsutus; Aziridine
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