Screening, Production and Optimization of Protease Enzyme from Streptomyces Species

P. Bosco Dhanaseeli and V. Balasubramanian

Centre for Ocean Research, AMET University,Kanathur,Chennai - 603 112, India.

DOI : http://dx.doi.org/10.13005/bbra/1433

ABSTRACT: In the present study Streptomyces species were screened as protease producer Based on the zone of clearance.The enzyme production was optimized using the following parameters like substrate, concentration, different temperature, pH, carbon source and Nitrogen source. The study was done up to 10 days, the crude enzyme was concentrated using Ammoniumsulphate precipitation, dialysis and Lyophilization.The substrate concentration 0.2 of casein was suitable for more protease Production and protein induction. At different pH, the study of pH 7 is favorable for more protease Production 6th day. It is also enhance the protein content According to the temperature study at 30 c of incubation it favor more Protease production and more protein content at 6th day Dextrose (2%) is the best carbon source for the more amount of protease Production and protein content at 10th day Peptone in the most favorable nitrogen source for protease production and protein content. The concentrated enzyme band patterns were observed on SDSPAGE.

KEYWORDS: Protease; Streptomyces; SDS-PAGE; Lyophilization; Precipitation; pH; Dextrose

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