In vitro Propagation of Ornamental Hybrids of Populus L

Anna Alekseevna Erst¹, Viktor Timofeevich Bakulin², Andrey Sergeevich Erst³, Alexander Alexandrovich Kuznetsov⁴, and Eugene Zhumanovich Bayahmetov⁵

¹Central Siberian Botanical Garden of the Siberian Branch of Russian Academy of Sciences, Zolotodolinskaya str., 101; 630090, Novosibirsk, Russia; Altai State University, Lenina st. 61; 656049, Barnaul, Russia. ²Central Siberian Botanical Garden of the Siberian Branch of Russian Academy of Sciences, Zolotodolinskaya str., 101; 630090, Novosibirsk, Russia. ³National Research Tomsk State University, Laboratory of Biodiversity and Ecology, prospekt Lenina, 36; 634050, Tomsk, Russia; Central Siberian Botanical Garden of the Siberian Branch of Russian Academy of Sciences, Zolotodolinskaya str., 101; 630090, Novosibirsk, Russia. ⁴National Research Tomsk State University, Laboratory of Biogeochemical and of Remote Methods of Monitoring Environmental, prospekt Lenina, 36; 634050, Tomsk, Russia. ⁵National Research Tomsk State University, Laboratory of Biodiversity and Ecology, prospekt Lenina, 36; 634050, Tomsk, Russia

DOI : http://dx.doi.org/10.13005/bbra/1442

ABSTRACT: To propagate two hybrids of the genus Populus (Siberian silver poplar #12 and «Strojnyi» poplar #18/4) and two species (P.alba and P.nigra) the culture technique for plant tissues and organs in vitro was used. The following ways of morphogenesis in vitro were revealed: direct (adventitious shoot-forming, development of axillary buds) and indirect (bud formation). The dependence of explants response on the maternal plant genotype was shown. The best morphogenic response was obtained for Populusnigra and P.alba (72% to 100%). The key factor for the shoots developing from the callus culture is the introduction of gibberellic acid into the medium. At the stage of shoot multiplication the best media are Murashige, Skoog and Gamborg, Eveleigh supplemented by 6- benzylaminopurine 1μM and gibberellic acid 5μM. The microshoots obtained by both direct and indirect morphogenesis were successfully rooted on the double reduced Murashige, Skoog medium. To propagate and preserve the unique poplar hybrids (Siberian silver poplar #12 and «Strojnyi»poplar #18/4) we recommend the direct morphogenesis as the most efficient in its parameters and providing better genetic stability of the regenerated plants.

KEYWORDS: Populus; hybrids; in vitro propagation; morphogenesis

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