Effect of Carbon Sources and Sucrose Concentrations on Shoot Organogenesis of Aloe Saponaria

Jae Kwang Kim¹, Thanislas Bastin Baskar² and Sang Un Park^2*

¹Division of Life Sciencesand Bio-Resource and Environmental Center, Incheon National University, Incheon 406772, Korea.

²Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-Gu, Daejeon, 305-764, Korea.

Corresponding Author E-mail: supark@cnu.ac.kr

DOI : http://dx.doi.org/10.13005/bbra/2116

ABSTRACT: In the present study, the effect of various carbon sources and sucrose concentrations on in vitro organogenesis of Aloe saponaria was investigated and a rapid micropropagation protocol was developed from in vitro-derived meristem explants. Meristem explants were cultured in initial shoot regeneration media with five different carbon sources (fructose, glucose, lactose, maltose, and sucrose), and sucrose as the best carbon sources for shoot regeneration and shoot elongation was investigated at five different concentrations (10, 20, 30, 40, 50 mg L^-1). The treatment with sucrose resulted in the highest number of shoots (2.7 ± 0.2) per explant and produced the longest shoots (16.4 ± 1.3 mm), whereas the treatment with maltose was the least efficient in promoting shoot number (1.5 ± 0.1) and shoot elongation (10.4 ± 0.9 mm). The highest shoot regeneration (3.3 ± 0.3) and the longest shoots (19.1 ± 1.5 mm) were observed in treatments with 40 g L^-1 sucrose. Further increase in sucrose concentration delayed shoot induction, resulting in stout shoots stunted in their growth. Our results suggest that carbon sources, particularly sucrose, could be used for micropropagation and in plant transformation protocols for regeneration of Aloe species.

KEYWORDS: Aloe saponaria; Shoot organogenesis; Fructose; Glucose; Lactose; Maltose; Sucrose

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