An Efficient Method for in vitro Plant Regeneration and Micropropagation of Aloe saponaria

Sook Young Lee¹, T. Bastin Baskar², Haeng Hoon Kim³, Jai-Heon Lee^4* and Sang Un Park^2*

¹Regional Innovation Center for Dental Science & Engineering, Chosun University, 309 Pilmun-daero, Dong-gu, Gwangju, 501-759, Korea.

²Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon, 305-764, Korea.

³Department of Well-being Resources, Sunchon National University, 413 Jungangno, Suncheon, Jeollanam-do, 540-742, Korea.

⁴Department of Genetic Engineering, Dong-A University, Busan 604-714, Korea.

DOI : http://dx.doi.org/10.13005/bbra/2164

ABSTRACT:

Aloe has traditionally been used as a folk medicine,although it is currently used as a component of various globally available commercial products. In this study, we investigated a method to improve the in-vitro regeneration and micropropagation of Aloe saponariausing 2-year-old meristem explants. After sterilization,explants were cultured in different basal media (MS,B5,SH) containing 2mg/L 6-benzylaminopurine (BAP). We found thatthe optimal basal medium was MS with 2mg/L BAP, which produced the greatest number of shoots per explant.We also investigated the effect of MS media at different concentrationson shoot regeneration and length, and found the best conditions to be 1MS with 2mg/L BAP. Further, we examined the effects of using different concentrations of the gelling agents Phytagar and Gelrite and found that 3mg/L Gelritewith 2mg/l BAP regeneratedthe highest number of shoots. Micropropagation of Aloe saponaria was improved with the addition of various concentrations of activated charcoal (AC), where more shoots of greater length were obtained with 1g/L AC in MS containing 2mg/L BAP. These results demonstrate that the micropropagation of aloe can be improved, and that using meristem explants is an effective method for plant regeneration.

KEYWORDS: Plant regeneration; Aloe saponaria; micropropagation; meristem explants

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