Prevalence and Molecular characterization of Circulating Respiratory Syncytial Virus (RSV) in Chennai, South India during 2011-2014.

B. V. Suresh Babu ^1,2, P.Gunasekaran ¹ ,P.Venkataraman  ², ,S. Mohana ¹, R. Kiruba  ¹, K. Ruban ¹, S. Magesh  ¹, CP. Indhumathi ¹,C. P. Anupama¹, A. K. Sheriff  ¹, Kavita Arunagiri ¹ and K. Kaveri ^1*

¹Department of Virology, King Institute of Preventive Medicine and Research, Guindy, Chennai, Tamilnadu, India.

²Department of Medical research, SRM Medical college hospital and Research Centre, SRM University, Chennai, Tamilnadu, India.

Corresponding Author E-mail: kinginstitutevirology@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/2132

ABSTRACT: The objective of the study was to determine the prevalence and Genotyping of RSV A and B in Chennai, Tamilnadu, south India. Human respiratory syncytial virus (RSV) is one of the most important respiratory viruses causing acute respiratory tract infections amongst children. Based on genotyping of the attachment glycoprotein (G) gene, it is divided into two groups, RSV-A and RSV-B. We tested 850 samples of patients with influenza like illness (ILI) severe respiratory illness (SARI) during the period 2011-2014 for RSV using a conventional RT-PCR and found 124 (14.5%) samples to be positive for RSV. Further sub typing by nested PCR in which 84(10%) were RSV A, 40 (4.5%) were RSV B, suggest that RSV-A was the predominant group circulating in south India during the study period.  Among  patients  with RSV  infection,  47.5%  were   less  than 1 year age group, 29.8 %  were between  1-5 year age group, 14.5% were between 6 to 14 years age group and  8 % were above 14 years age. Phylogenetic analysis all RSV-A sequences belonged to ON1 within NA1 genotype RSV-B sequences belonged to the genotype BA9 and BA12.

KEYWORDS: Respiratory Syncytial Virus; RSV infections in Chennai; RT-PCR for RSV;RSV Genotyping in India

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