Aloe Vera’s Effect on Acetyl Cholinesterase(AChE) and Ultra Structure of Motoneurons in Synaptic Zone After Spinal Cord Injury in Rats

Marjan Heshmati^1*, Mohammad Reza Jalali²,Nadoushan³ and Maryam Sharayeli³

¹Department of Anatomical Sciences and Patholog ,Faculty of Medicine, Shahed University,Opposite Holy Shrine of Imam Khomeini Khalij Fars Expressway Tehran, Iran.

²Mostafa Khomeyni Hospital,Faculty of Medicine,Shahed University,Tehran,Iran.

³Department of Anatomical Sciences and Patholog ,Faculty of Medicine ,Shahed University,Opposite Holy Shrine of Imam Khomeini Khalij Fars Expressway Tehran, Iran.

Corresponding Author E-mail: heshmatimarjan@hotmail.com

DOI : http://dx.doi.org/10.13005/bbra/2119

ABSTRACT: Spinal cord injury(SCI) followed by transection, contusion or compression areconsidered to be similar to human SCI.Nowadays researchers demonstrated alternativetherapies as pharmacological properties or herbs to repaire these damage.Aloe Vera is known as a plant with multi potential activity.We used this plant after SCI by Clips Aneurysm(30g /1 min). 32 female Sprague Dawley rats were divided randomly into four groups.Group 1: Sham( laminectomy) + gavage Distilled water, group2: Sham +gavage Aloe Vera gel powder(200mg/kg/d), group3: group 2+SCI, and group4:group 1+SCI (N=8).After 4 weeks they were sacrificed.Morphometric study and Acetyl cholinesterase(AChE) immune staining were done.Synaptic changes was analyzed in a blinded manner for qualitative ultra structural changes.The data analyzes with Tukey’s test and one-way ANOVA in SPSS21 software and P≤0.05 was considered as significant level.Decreasing motoneurons ,reducing AChE immune activity with ultrastructure changes in mitochondria and synaptic zone were seen due to SCI.Usage of Aloe Vera gel powder demonstrated a reduction in death of motoneuron(Pd”0.05) with increasing AChE immunoreactivity after SCI(Pd”0.05).This herb affected on ultrastructure as seen pathological synaptic changes and mitochondria decreases.Aloe Vera gel powder effects might be due to it’s antioxidant which has reduced neuronal cell death and preserved damaged neurons as intact neurons.

KEYWORDS: Aloe Vera;Spinal Cord Injury;Synapse;Rat

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