The in Vitro Effect of Different Cord Blood Platelet Rich Plasma Concentrations on Proliferation of Dermal Fibroblasts

Hashemi S. S¹, Servatkhah M.¹ and Rafati A. R²

¹Burn and Wound Healing Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran.

²Division of Pharmacology and Pharmaceutical Chemistry , Sarvestan Branch, Islamic Azad University ,Sarvestan, Iran.

Corresponding Author E-mail: alireza_rafati57@yahoo.com

DOI : http://dx.doi.org/10.13005/bbra/2321

ABSTRACT: platelet-rich plasma (PRP) is a source of a lot of growth factors and other soluble proteins that can modulate tissue regeneration. Skin fibroblasts play a main role in tissue remodeling and wound repairing. We decided that the human umbilical cord blood platelet-rich plasma (CPRP) affect fibroblast proliferation and cell migration assay. A fibroblast culture was established from a neonatal foreskin. Various concentrations of PRP were prepared from human umbilical cord blood sources and applied to primary human dermal fibroblasts. Cell proliferation was measured by MTT assay. We showed that human umbilical cord blood platelet-rich plasma stimulate the proliferation and migration of dermal fibroblasts and that stimulation by CPRP is dose dependent. human umbilical cord blood platelet-rich plasma can be used to treat chronic skin defects, without triggering an immune response.

KEYWORDS: Human umbilical cord blood; Plasma Rich Platelet; proliferation; migration; human skin fibroblast

[ HTML Full Text]