Production of Bioethanol from the Hydrolysate of Brown Seaweed (Sargassum Crassifolium) using A Naturally Β-Glucosidase Producing Yeast Saccharomyces Cereviceae JCM 3012

Trianik Widyaningrum^1,2, Indro Prastowo*^1,4, Masreza Parahadi³ and Angga Dwi Prasetyo³

¹Department of Biology Education, Faculty of Teacher Training and Education, Ahmad Dahlan University, Kampus III, Jl. Prof. Dr.Soepomo, Janturan, Yogyakarta 55164, Indonesia.

²Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Gedung Biologi, Jl. Veteran, Malang 65145, Indonesia.

³Department of Postgraduate Program in Biotechnology, Postgraduate School, Gadjah Mada University, Jl. Teknika Utara, Barek Yogyakarta 55281, Indonesia.

⁴Nitidipuran Life Science Studio, Jl. Nitidipuran 26, Ngupasan, Gondomanan, Yogyakarta 55122, Indonesia.

DOI : http://dx.doi.org/10.13005/bbra/2274

ABSTRACT: The efforts to produce bioethanol using non-crop raw material are recently developed. Sargassum crassifolium is a promising raw material for biofuel production since containing a high concentration of polysaccharides. Pre-treatment of seaweed at working pressure of 15 Psi, temperature of 121 ^oC and sulphuric acid concentration of 0.2 M increased the reducing sugars concentration up to 26.68 g/l. The application of cellulase (0.5 g/ml) helped increasing the reducing sugars concentration up to 2.56 times (increasing up to 68.32 g/l). Fermentation using a naturally β-glucosidase producing yeast, Saccharomyces cereviceae (JCM 3012) (0.15 g/ml), produced the final bioethanol conversion up to 90.99%. The activity of β-glucosidase increased up to 20.39 U/ml during fermentation that assisted the degradation of seaweed.

KEYWORDS: Bioethanol; cellulase; seaweed Sargassum crassifolium; Saccharomyces cereviceae JCM 3012; β-glucosidase

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