Volume 13, number 3
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Purification and Characterization of Neutral Protease from Bacillus Substilis UBT7 Isolated from Terasi, Indonesian Fermented Fish

Asep A. Prihanto1, Abdul A. Jaziri1 and Ima Y. Perwira2

1Department of Fishery and Marine Science, Faculty of Fisheries and Marine Science, Brawijaya University, Veteran street, Malang, 651245, East Java Indonesia.

2Department of Aquatic Resources Management, Faculty of Marine Science and Fisheries, Udayana University, Bali - Indonesia.

Corresponding Author E-mail: asep_awa@ub.ac.id

DOI : http://dx.doi.org/10.13005/bbra/2283

ABSTRACT: Neutral protease producing bacterium Bacillus substilis UBT7 strain isolated from Terasi, an Indonesian fermented fish grew in shake flask and fermenter, and produced protease at 37 °C, pH 7 for 24 hour. After three purification steps, the enzyme was successfully purified. This neutral protease had an apparent molecular mass of 32 kDa as estimated by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis. The enzyme was active optimum on 50 °C. This indicated that the enzyme was slightly thermozyme. The stability was decreased on 40 °C. The enzyme was optimum in pH 7. The enzyme was somehow enhanced with Fe2+ and K2+ metal ionsbut not by the addition of Ni2+.

KEYWORDS: Terasi; Bacillus substilis UBT7; neutral protease; fermented fish; Indonesia  

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Prihanto A. A, Jaziri A. A, Perwira I. Y. Purification and Characterization of Neutral Protease from Bacillus Substilis Ubt7 Isolated from Terasi, Indonesian Fermented Fish. Biosci Biotech Res Asia 2016;13(3).
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Prihanto A. A, Jaziri A. A, Perwira I. Y. Purification and Characterization of Neutral Protease from Bacillus Substilis UBT7 Isolated from Terasi, Indonesian Fermented Fish. Biosci Biotech Res Asia 2016;13(3). Available from: https://www.biotech-asia.org/?p=15709

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