Isolation and Characterization of Rhizobacteria from Mustard Field

M. S. Bochalya, Anil Kumar, A. S. Rathi, Rakesh* and Kushal Raj

Department of Plant Pathology CCS Haryana Agricultural University- Hisar.

Corresponding Author E-mail: punia.rakesh98@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/2395

ABSTRACT: An in vitro study were carried out in the Department of Plant Pathology, CCS Haryana Agricultural University to isolate and identify different rhizobacterial isolates present in mustard field (cv RH-30). Samples of soil, rhizoplane and rhizosphere were collected from previously grown mustard fields of pathological research farm. The rhizobacterial isolates were isolated on King’s ‘B’medium by serial dilution of samples. These isolated rhizobacterial isolates were identified by following standard protocols. Results indicated that in mustard field three species of genus Bacillus, three species of genus Rhizobium, two species of genus Pseudomonas, one species of genus Arthrobacter and one species of genus Micrococcus were found. All three species of genus Bacillus having same morphological, physiological and biochemical characters except colony colour (white and yellow), colony thickness (large, medium and small raised), colony smoothness (smooth and rough), colony arrangement (lobate, entire and waxy) bacteria arrangement (pair and chains), citrate utilization test (+ve and -ve), oxidase test (+ve and -ve) and acid production test (+ve and -ve). Whereas Rhizobium genus are different in colony colour (white and pink), colony thickness (large and medium raised), bacterial morphology (medium, long and big rods), bacteria arrangement (single and groups), indole production test (+ve and -ve), methyl red test (+ve and -ve), citrate utilization test (+ve and -ve), oxidase test (+ve and -ve), starch hydrolysis test (+ve and -ve)and cellulose hydrolysis test (+ve and -ve). Two species of genus Pseudomonas is different in colony colour (yellowish white and creamy white), colony appearance (flat and convex), bacterial morphology (small and medium rods) and methyl red test (+ve and -ve). After identification these rhizobacterial isolates were maintained on King’s ‘B’medium for further studies like their possible role in induction of systemic resistance against Sclerotinia sclerotiorum causing stem rot in mustard alone or in combination with different non-conventional chemicals (salicylic acid, acetyl salicylic acid, indole butyric acid, indole acetic acid, zinc sulphate, magnesium sulphate and carbendazim fungicide at 10, 50 and 100 ppm). Effect of these isolated rhizobacterial isolates and non-conventional chemical on biochemical composition and enzymatic activity will also be tested.

KEYWORDS: Rhizobacteria; Mustard; Rhizoplane; Rhizosphere; Pseudomonas; Bacillus; Rhizobium

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