Characterization and Identification of Seed Storage Protein of Twelve Lettuce Cultivars

Sameer Hasan Qari¹, Ehab Abdel-Razik Kamel¹ and Hameda El Sayed²

¹Biology Department, Aljamom University College, Umm Al-Qura University, P.O. Box: 2064, Makkah, Saudi Arabia.

²Biology Department, Faculty of Applied Science, Umm Al Qura University, Makkah, Saudi Arabia.

 Corresponding Author E-mail: sqarinet@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/2350

ABSTRACT: We used SDS-PAGE to evaluate and characterize the protein patterns of seed storage proteins in 12 lettuce cultivars. Total protein content of lettuce seeds in all cultivars did not show any significant difference. Results of SDS- PAGE pattern of a few protein bands were up regulated whereas some other bands showed down regulation. The identified protein patterns may be used protein marker for lettuce cultivars. The seed storage protein analyses helps in characterization and identification of diversity in lettuce crop varieties, cultivars and their wild varieties and also provides information on phylogenetic relationship of the accessions. It is also known that variation in protein bands provide information on the relationship among the used seeds. SDS-PAGE of seed protein using Tris-glycine buffer was performed to study the relationships within 12 cultivars of Lactuca sativa L. (lettuce). In the present study, SDS-PAGE was used to investigate and characterize the protein patterns of seed storage proteins in order to find protein bands as markers for cultivar characterization. Datawereanalyzedby clustering method and similarity coefficients using NTSYSpcversion2.02i. ThevalidityofclusteranalysisofSDS-PAGE seed proteins profile datawasdiscussed.

KEYWORDS: SDS-PAGE; Seed Protein; Lettuce; (Lactuca sativa); Cultivars; Numerical Analysis; Cluster Method; Relationships

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