Molecular Characterization of Lactic Acid Bacteria Isolated from Rumen Liquor of Goat

Gothandam Ladha and Kadirvelu Jeevaratnam*

Department of Biochemistry and Molecular Biology, Pondicherry University, R.V.Nagar, Kalapet-605 014, India.

Corresponding Author E-mail: jeevskj@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/2378

ABSTRACT: Objective of this study is to isolate and molecular characterise lactic acid bacteria from rumen liquor of goat. A total of 58 isolates, 42 bacilli and 16 cocci on gram staining were obtained. Screening for antimicrobial activity using cell free supernatant resulted in 12 potentisolates, 9 bacilli and 3 cocci exhibiting wide range spectrum of antimicrobial activity against food borne pathogens. The antimicrobial activity was lost on treatment with protease suggesting the isolates bacteriocinogenic in nature. These potent bacteriocinogenic isolates were characterized by physiological and biochemical properties including carbohydrate utilization profile and RAPD profile using M13 and R2 primers to differentiate at strain level. The 16 rRNA gene sequence analysis coupled with multiplex PCR analysis using recA gene amplification ther isolates were identified as Lactobacillus plantarum (7), Lactobacillus fermentum (2) and Pediococcus pentosaceus (3). Further work on their probiotic and other beneficial properties may lead to several applications and benefit to animal and humans.

KEYWORDS: Rumen liquor; Pediococcus; Lactobacillus; RAPD; 16S rRNA

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