Isolation and Characterization of cellulose Producing Bacterial isolate from Rotten grapes

Omchand Singh¹, Parmjit S. Panesar¹ and Harish K. Chopra²

¹Biotechnology Research Laboratory, Department of Food Engineering and Technology, Sant Longowal Institute of Engineering and Technology, Longowal-148106, Punjab, India.

²Department of Chemistry, Sant Longowal Institute of Engineering and Technology, Longowal-148106, Punjab, India.

Corresponding Author E-mail: pspanesarrr@yahoo.com

DOI : http://dx.doi.org/10.13005/bbra/2455

ABSTRACT: In this present investigation, eleven bacterial cellulose producing isolates were selected out of 34 isolates from different sources. Among the eleven isolates the C18 isolate has been identified as a potential bacterial cellulose producer. Physiological and biochemical tests were carried out to identify the bacteria. The molecular characterization of C18 strain was done by 16 S rDNA analysis and identified as Gluconacetobacter xylinus due to 94% sequence similarity. The maximum bacterial cellulose production (3.96g/L) was obtained after incubation time of 168 h with Hestrin and Hchramm (HS) media in static culture.  Structural elucidation of bacterial cellulose was achieved using analytical techniques like FTIR, SEM and X-ray diffraction analysis.

KEYWORDS: Gluconacetobacter xylinus; bacterial cellulose; FTIR; SEM; X-ray diffraction

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