Biotin Axotroph Mutants of Native Corynebacterium Glutamicum for the Fermentative Production of L-Glutamic Acid

V. Krishnarjuna Reddy¹, U. Keerthi¹, A. V. N. Swamy² and Dowlathabad Muralidhara Rao¹

¹Department of Biotechnology, Sri Krishnadevaraya University, Ananthapuramu, India.

²Department of Chemical Engineering, Jawaharlal Nehru Technological University Ananthapuram, Ananthapuramu, India.

Corresponding Author E-mail: rao.muralidhara@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/2460

ABSTRACT:

The present paper deals with isolation of C.glutamicum strain and strain improvement of C.glutamicum by mutation studies for the production of L-Glutamic acid. Native isolate of C.glutamicumwas manipulated to excrete L-glutamate. The modified protocol was used in this paper to trigger L-glutamate excretion. The important feature of this protocol is to achieve a biotin concentration that is low enough to activate glutamate excretion but high adequate to still allow sufficient growth of the cells. To achieve this appropriate biotin concentration, a pre cultivation step is required in order to deplete the cells of biotin. Later Physical Mutation was induced on Biotin depleted C.glutamicum strain.

KEYWORDS:

Biotin;Corynebacterium glutamicum: Fermentation L-Glutamic acid;

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