Effect of Surface Sterilization time and Plant Bioregulators for Callus Formation in Hybrid Lilium Cv. Tresor

Anchal Arpita Gochhayat¹, Sashikala Beura² and Enketeswara Subudhi¹

¹Centre of Biotechnology, Siksha O Anusandhan University, Bhubaneswar-751003, India.

²Biotechnology-cum-Tissue culture Centre, Orissa University of Agriculture and Technology, Bhubaneswar-751003, India.

Corresponding Author E-mail: sashikalabeura@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/2498

ABSTRACT: An efficient protocol was standardized for calli mass formation from bulb scale explant of hybrid  Lilium Cv. Tresor under in vitro conditions at Biotechnology-cum-Tissue Culture Centre, OUAT, Bhubaneswar. The bulb scale explants were treated with   0.1 % HgCl₂ (3 min, 4min, 5min, 6 min, 7min, 8min and 9 min) and  control (without treatment) were cultured on MS media, among the treatments, 5 minutes timing  resulted in minimum contamination [fungal % (6.67), bacterial % (6.67)] and maximum survival % (83.33%). The best surface sterilization time was further taken into consideration for treatment of explants, sterilization and  cultured in the MS Basal media supplemented with  BAP (0.5, 1.0 mg/l) in combination with 2,4-D (0.5,1.0,1.5,2.0, and 2.5 mg/l) and 2,4-D (0.5,1.0,1.5,2.0, and 2.5 mg/l)  alone along with control. Basal media supplemented with BAP (1.0 mg/l) and 2,4-D (1.50 mg/l) produced maximum callus %  (90.00%) and spread, profuse green callus was also recorded in similar combination which opened prospects for developing an indirect means of in vitro regeneration of hybrid Lilium Cv. Tresor there by strengthening the way biotechnology which could be used for improvement and satiate the national and international demands of this cut flowers.

KEYWORDS: Bacterial; Bulb; Fungal; HgCl₂; Callus

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