Investigation on the Effect of Carbon and Nitrogen Sources for the Production of Cellulosome by Trichoderma Reesei NCIM 1186 Using Saturated Placket Burman Design

Preethi Jampala, Preethi Murugan, Swathy Ramanujam and Kiran Babu Uppuluri*

Bioprospecting Laboratory, Department of Biotechnology, School of Chemical and Biotechnology, SASTRA University, Thanjavur-613401, Tamil Nadu, India

ABSTRACT: Lignocellulosic materials are abundant in nature but cannot be readily utilised as substrates for the fermentation due to their complexity. Breakdown of plant cell wall into easily utilisable substrates can be done by usage of enzymes like cellulases, xylanases and related enzymes. Cellulosome is a macromolecule which is a complex of such enzymes that act synergistically on lignocellulosic materials for complete degradation. Cellulosome have impressive applications in biotechnology as conversion of cellulosic biomass into simple sugars can be useful in the production of high – value products like organic acids and ethanol from inexpensive and renewable sources.  Till date only some bacteria have shown the ability to produce cellulosomes. In present study, Trichoderma reesei NCIM 1186, cellulolytic fungi has been studied for its ability to produce cellulosome in Prosopis juliflora pods containing medium. In view of enhancing the cellulosome production, the effect of various carbon and nitrogen sources supplementation was studied using a statistical method, saturated Plackett – Burman (PB) design which is a modified version of PB. Seven carbon sources and eleven nitrogen sources were screened for their significant effect on the production of cellulosome. Out of tested carbon and nitrogen sources, maltose and ammonium hydrogen carbonate shown a significant effect on cellulase activity and maltose and ammonium sulphate shown a significant activity on xylanase activity of cellulosome.

KEYWORDS: Trichoderma reesei; Cellulosome; Saturated Plackett-Burman design; Prosopis juliflora

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