An Efficient Protocol for Shoot Organogenesis and Plant Regeneration of Polygonummultiflorum

Woo Tae Park¹, Sang Un Park¹, Sun Kyung Yeo,¹ Md Romij Uddin², Haeng Hoon Kim³

¹Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon, 305-764, Korea ²Department of Agronomy, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh. ³Department of Well-being Resources, Sunchon National University, 413 Jungangno, Suncheon, Jeollanam-do, 540-742, Korea  

ABSTRACT: We developed a rapid protocol for efficient shoot organogenesis and plant regeneration from stem node cultures of Polygonummultiflorum. Stem node explants were cultured with varying concentrations of cytokinins, then with 0.5 mg/l of 6-benzylaminopurine (BAP) supplemented with various concentrations of different auxins, and regeneration capacity and growth of shoots were assessed. Among the cytokinins, BAP induced shoot initiation with the highest efficiency, whereas zeatin showed higher efficacy for stimulation of shoot growth after 6 weeks in culture. BAP with indole-3-acetic acid (IAA) was highly effective in stimulating shoot initiation. Among the treatment combinations, 0.5 mg/l BAP with 1.0 mg/l IAA proved optimal yielding the highest number of shoots (3.1 per explant). This system can produce approximately 4 plantlets in vitro from one stem node explant culture of P. multiflorum, and the system can be used at any time of the year.

KEYWORDS: plant regeneration; Polygonummultiflorum; shoot organogenesis

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