A Preliminary Investigation of Viral Pathogen Causing Foetal Abortion in Donkeys at Ethiopia

Uzoamaka Adaobi Okoli

Department of Biomedical Sciences, School of Health and Bioscience, University of East London, Stratford Campus, Water Lane, London E15 4LZ.

Corresponding Author E-mail: Uzoamaka.okoli@unn.edu.ng

DOI : http://dx.doi.org/10.13005/bbra/2542

ABSTRACT: Rabbit kidney (RK13) cells infected with virus isolated from a homogenate made from the placenta of aborted foetal donkeys were stained with Leishman’s stain, morphological changes including cytopathic effects (CPE), syncytia, and inclusion bodies were seen by light microscopy after incubation for 24hrs-96hrs at 37⁰C.  After 48hrs of incubation, about 60% of cells were infected. Another Set of RK 13 cells infected with either native virus or both ether treated virus and native virus in the presence of acyclovir was stained with Giemsa, morphology changes were observed in the native virus infected cell while little or no change was seen in infected cells in the presence of acyclovir and ether treated virus respectively. Virus infected RK13 cells were stained with acridine orange, intracellular fluorescent green colour was seen by fluorescence microscopy in the cell nucleus. The clinical history and CPE of the virus in RK13 cell are similar to Equine Herpes virus.

KEYWORDS: Acyclovir;  Cytopathic Effects Fluorescence Microscopy; Rabbit Kidney Cells;

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