Volume 14, number 4
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Isolation, Identification and Characterization of Streptomyces Sp. SN-2

Bantanahal Talari Vishwanatha1, Girish Babu K2, Padmashri1, Swathi B Malagi1, Chethan J Dandin2 and Sreenivasa Nayaka1

1Department of Botany, Karnatak University, Dharwad-580003, Karnataka, India.

2Department of Microbiology and Biotechnology, Karnatak University, Dharwad-580003, Karnataka, India.

Corresponding Author E-mail: sreenivasankud@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/2585

ABSTRACT: The present investigation is carried out for the isolation and molecular characterization of actinomycetes from the soil samples from Karnatak University campus, Dharwad, Karnataka, India. Totally four actinomycetes isolated, out of four isolated only one actinomycetes strain (SN-2) showed positive results for antimicrobial activity. SN-2 isolated actinomycetes was further analysed for morphological, physiological, biochemical and 16S ribosomal RNA gene sequencing. The SN-2 strain gene sequence was predicted with secondary structure and restriction sites were identified with the help of Genebee and NEBcutter tools. This investigation clearly indicates that the isolated strain SN-2 as Streptomyces sp.

KEYWORDS: Actinomycetes; Streptomyces; Antimicrobial; 16S rRNA; Cross streak method

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Vishwanatha B. T, Babu K. G, Padmashri P, Malagi S. B, Dandin C. J, Nayaka S. Isolation, Identification and Characterization of Streptomyces Sp. SN-2. Biosci Biotech Res Asia 2017;14(4).
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Vishwanatha B. T, Babu K. G, Padmashri P, Malagi S. B, Dandin C. J, Nayaka S. Isolation, Identification and Characterization of Streptomyces Sp. SN-2. Biosci Biotech Res Asia 2017;14(4). Available from: https://www.biotech-asia.org/?p=28072

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