Antimicrobial Activity and Biochemical Profiling of Selected Medicinal Plants Against Blood Cancer Clinical Isolates

Afnan Abdul-Jalil Farhan¹, Munazza Gull¹, Sawsan Abdulaziz Rahimuddin¹, Taha Abdullah Kumosani¹, Ahmed Mahmoud Al-Hejin², Abida Kausar³ and Muhammad Aamer Mehmood⁴

¹Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.

²Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.

³Department of Botany, Faculty of Science, Govt: College University (Women) Faisalabad, Pakistan.

⁴Department of Bioinformatics and Biotechnology, Government College University Faisalabad, 38000, Pakistan.

Corresponding Author E-mail: munagull@hotmail.com

DOI : http://dx.doi.org/10.13005/bbra/2570

ABSTRACT: The need of antibiotics obviate in treated cancer patients when suppression of immune system leads to secondary infections development. The objective of the present study was to evaluate the antibacterial activity and biochemical profiling of various medicinal plants Trigonella foenum-graecum, Ocimum basilicum, Olea europaea, Mentha longifolia and Boswellia sacra against clinical isolates of blood cancer cases. Crude plant extracts in ethanol and methanol were used to test antimicrobial activity through disc diffusion method. Biochemical profiling identified the presence of Gallic acid, parahydroxy benzoic acid, vanillic acid, syringic acid and ferulic acid by high performance liquid chromatography (HPLC). Boswellia sacra showed the maximum antibacterial activity against Streptococcus viridian with 12.4 mm inhibition zone. Trigonella foenum-graecum showed the maximum antibacterial activity against Salmonella Group B 11.8 mm with crude extracts in methanol. The antibacterial activity showed that Streptococcus viridian and Corynebacterium were more inhibited bacteria but Klebsiall pneumonia was found more resistant. Total phenolics analysis by HPLC revealed that parahydroxy benzoic acid was the major phenolic acid found in Olea europaea with 797.8 ng/g. The highest concentration of Gallic acid was found in Ocimum basilicum with 547.02 ng/g. These results indicated that these medicinal plants may serve as antimicrobial agents against clinical bacterial isolates from cancer patient successfully.

KEYWORDS: Antibiotics; HPLC; Leukemia; Plant Extract;Phenolic Acids

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