Propagation of Rootstocks of Gisela 5 Based on Tissue Culture Method

Zahra Tariverdi¹, Kamran Akbari Nughabi² and Saeed Piri³

¹Gardening/Biotechnoly and molecular genetics of gardening products, Abhar Branch, Islamic Azad University, Zanjan, Iran.

²Gardening, Faculty of Gardening, Abhar Branch, Islamic Azad University, Zanjan, Iran.

³Fruit Culture, Faculty of Gardening, Abhar Branch, Islamic Azad University, Zanjan, Iran.

Corresponding Author E-mail: zahratariverdi96@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/2584

ABSTRACT: Cherry is one of the most common fruits in the moderate conditions. Common rootstocks like Gisela5 have been used in the gardening field in recent years. The purpose of the current study is investigating the enhancement of Gisela5 rootstock method on the basis of tissue culture. Cutting grafting was used as explant material. These explants included the following materials in the MS environment: 1mg/l IBA (Indole-3-butyric acid), 0.75 mg/l BPA, 1mg/l BPA+ 1 mg/l BPA, 0.75 mg/l BPA + 2mg/l BPA and 1mg/l BPA +2mg/l BPA. The planting was kept in the planting room with 24 ±2 Oc temperature. The basic environment of MS completed with 0, 1,2,4,6 mg/l NAA during the rooting stage. The maximum number of branches which was 289 was achieved by means of the combination of the following values: 1 mg/l INA+ 0.75 mg/l BAP and 2mg/l IBA +1 mg/l BAP. The maximum length of the branches was 1.69 cm. varieties of concentrations of NAA were added in the rooting stage. The amount of rooting was increased by adding almost 6 mg/l of NAA into the rooting stage

KEYWORDS: Cherry;Gisela 5; Rootstocks; Tissue Culture

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