Cold Active Amylases Producing Psychrotolerants Isolated from Nella Lake, Antarctica

Abhas Kumar Maharana and Shiv Mohan Singh

Polar Biology Laboratory, National Centre for Antarctic and Ocean Research, Vasco-da-Gama, Goa-403804, India.

Corresponding Author E-mail: drshivmohansinghncaor@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/2603

ABSTRACT: Cold active amylase was investigated by bacteria and yeast isolates from the sediment core samples of Nella Lake,Larsemann Hills region, East Antarctica. Between potential yeast and bacteria isolates screened for amylases, best isolates were identified asRhodotorula sp. Y-37 and ArthrobacteralpinusN16 by molecular technique.Amylase production capabilities of both the isolate subjected for optimization processes by using submerged fermentation technique with soluble starch as substrate.The results indicate that a supplement of 1% w/v glucose, 1% w/v yeast extract and 0.1% w/v KCl at pH 7.0with 5% v/v inoculum enhances the amylase production by 5.72-fold using Rhodotorula sp. Y-37. In other hands, the activators are 1% w/v of galactose and peptone, 0.1% w/v KCl and 2.5% v/v inoculum at pH 7.0 enhances the amylase production by 3.74-fold using ArthrobacteralpinusN16. Cold-active amylasecan be used in detergent, textile, food and beverage industries. Bio-degradation of starchy materials by cold active amylases can contribute in cleaning of environment at cold regions without harming the climate.

KEYWORDS: Amylase; Arthrobacteralpines; Cold Active; East Antarctica; Larsemann Hills; Rhodotorula sp.

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