Isolation and Characterization of Abiotic Stress Tolerant Plant Growth Promoting Bacillus Spp. from Different Rhizospheric Soils of Telangana

K. Damodara Chari¹, R. Subhash Reddy², S. Triveni³, N. Trimurtulu⁴, CH. V. Durga Rani⁵ and M. Sreedhar⁶

^1,2,3Department of Agricultural Microbiology and Bioenergy, College of Agriculture, Professor Jayashankar Telangana State Agricultural University, R’nagar, Hyderabad-500030,Telangana ,India.

⁴Biofertilizer Production Laboratory, Agricultural Research Station, Amaravathi, Guntur-522020, Andhra Pradesh. India.

⁵Department of Molecular Biology and Biotechnology, Institute of Biotechnology, Professor Jayashankar Telangana State Agricultural University, Hyd-030.

⁶Quality Control Laboratory, Professor Jayashankar Telangana State Agricultural University, R’nagar, Hyd-030.

Crossponding Author E-mail: damuagmicro2012@gmail.com

DOI : http://dx.doi.org/10.13005/bbra/2653

ABSTRACT: Present investigation was carried out to identify plant growth promoting rhizobacterial isolates for abiotic stress tolerance. To achieve this bacterial isolates were isolated from different rhizospheric soils of Telanagana and screened for plant growth promoting properties and tolerance to different abiotic stresses such as pH, temperature, salt, drought and heavy metals. Such PGPR will be helpful for efficient management of abiotic stresses in crop production. Rhizospheric soils from normal, salt affected, drought affected and bulk soils were collected from different places of Telangana state. From all soil samples, based on cultural, morphological and biochemical characterization it was found that forty four were of Bacillus spp. Among the forty four (44) Bacillus isolates, twenty eight (28) isolates were showing plant growth promoting properties. These positive isolates tested for abiotic stress tolerance to pH, temperature, salt, drought and heavy metals (As and Cd). Four isolates were showed growth at pH range from 4-12 (BS 1, BS 3, BS 14, BS 18), five isolates were showed tolerance to 1.5 to 20 % of NaCl concentration (BS 1, BS 3, BS 14, BS 18, BS 42, six isolates showed tolerance to temperature from 20ºC -50ºC (BS 10, BS 14, BS 18, BS 27, BS 37, BS 43), four isolates showed tolerance to water potential from - 0.05 Mpa to- 0.73 Mpa (BS 4, BS 10, BS 18, BS 33).

KEYWORDS: Bacillus; Drought and Heavy Metal Tolerance PGPR; pH; Salt;  Temperature;

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